Primer

Part:BBa_K2982006

Designed by: Tsang Hoi Yeung, Ocean   Group: iGEM19_HK_GTC   (2019-10-15)


Forward primer with BBa_B0034 for amplifying BBa_E0040

A forward primer that contains RBS34 which is 60 base pairs long that can be used to amplify the GFP part BBa_E0040 by performing PCR. It can be used to characterise the RBS activity easily by measuring its fluorescence.


This will be used as a standard in measuring RBS activity as RBS strength is often measured relative to RBS34. This primer contains an XbaI restriction enzyme site, a 21-nucleotide random sequence, RBS34, another random sequence for binding, then the first 21 nucleotides of the GFP sequence

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None